FAQs

NGAL-frequently-asked-questions-1

You’ll find answers to all of the most commonly asked questions about NGAL testing and products below. If you can’t find the answer you’re looking for, please contact us.

 

 NGAL ELISA Assays

How do the NGAL detection kits work?
 The assay is a sandwich ELISA performed in microwells coated with a monoclonal antibody to NGAL. Bound NGAL is detected with another monoclonal NGAL antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin followed by the addition of a colour-forming substrate.
What is the format of the assay?
 Simple ELISA format – easy to perform. Ready-to-use calibrators and working solutions. Pre-coated ELISA strips All incubations performed at room temperature Automation possible on open ELISA workstations.
How should they be stored?
Store the kit with all reagents at 2-8°C. Do not freeze.
What is the range and sensitivity of the assay?
The range and sensitivity of the assays depends on the specific kit being used. This is detailed below:

KIT036 (human total NGAL)
Assay range: 10-1000 pg/mL
Assay time: 4 hours
Limit of detection :4 pg/mL

KIT 048 (human NGAL monomer)
Assay range 10-1000 pg/mL
Assay time <4 hours
Limit of detection 4 pg/mL

KIT042 (mouse total NGAL)
Assay range: 10-1000 pg/mL
Assay time: <4 hours
Limit of detection: <0.75 pg/mL

KIT046 (Rat total NGAL)
Assay range: 4-400 pg/mL
Assay time: <4 hours
Limit of detection: 0.5 pg/mL

KIT 043 (Dog total NGAL)
Assay range 4-400 pg/mL
Assay time <4 hours
Limit of detection 0.56 pg/mL

KIT 044 (Pig total NGAL)
Assay range 4-400 pg/mL
Assay time <4 hours
Limit of detection 1 pg/mL

KIT 045 (Monkey total NGAL)
Assay range 10-1000 pg/mL
Assay time <4 hours
Limit of detection 1.5 pg/mL

What is the total assay time?
The assay takes about 4 hours
Are the kits approved for diagnostic use?
The human NGAL ELISA kit (KIT 036) is CE-IVDmarked, but is also suitable for research uses. The kits for detection of animal ELISAs are for Research Use Only.
What sample types can be used?
The kits can be used for the in-vitro determination of NGAL in tissue fluids (e.g. plasma, serum or urine), tissue extracts or culture media.

About the NGAL Assay

Although NGAL is best known as being a biomarker of acute kidney injury (AKI), and is proving to be of great value in drug development in this context, it is now being implicated in as a key component of a number of other pathways and disease states. These include cancer, inflammation and sepsis.

Our sensitive and reliable ELISA based NGAL assays provide a reliable and sensitive means of measuring NGAL levels in both pharmaceutical and medical research settings.

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